Evaluation of 23S rRNA PCR Primers for Use in Phylogenetic Studies of Bacterial Diversity

The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly diffe...

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Detalles Bibliográficos
Main Authors: Hunt, Dana E., Klepac-Ceraj, Vanja, Acinas, Silvia G., Gautier, Clement, Bertilsson, Stefan, Polz, Martin F.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2006
Assuntos:
Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1393206/
https://ncbi.nlm.nih.gov/pubmed/16517676
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.3.2221-2225.2006
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