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PCR-Induced Sequence Artifacts and Bias: Insights from Comparison of Two 16S rRNA Clone Libraries Constructed from the Same Sample

The contribution of PCR artifacts to 16S rRNA gene sequence diversity from a complex bacterioplankton sample was estimated. Taq DNA polymerase errors were found to be the dominant sequence artifact but could be constrained by clustering the sequences into 99% sequence similarity groups. Other artifa...

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שמור ב:
מידע ביבליוגרפי
Main Authors: Acinas, Silvia G., Sarma-Rupavtarm, Ramahi, Klepac-Ceraj, Vanja, Polz, Martin F.
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2005
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317340/
https://ncbi.nlm.nih.gov/pubmed/16332901
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8966-8969.2005
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