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Validation of High-Resolution DNA Melting Analysis for Mutation Scanning of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Gene

High-resolution melting analysis of polymerase chain reaction products for mutation scanning, which began in the early 2000s, is based on monitoring of the fluorescence released during the melting of double-stranded DNA labeled with specifically developed saturation dye, such as LC-Green. We report...

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Detalhes bibliográficos
Main Authors: Audrezet, Marie-Pierre, Dabricot, Aurélia, Le Marechal, Cédric, Ferec, Claude
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2518737/
https://ncbi.nlm.nih.gov/pubmed/18687795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2008.080056
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