Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate

The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca(2+)-releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchlor...

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Autores principales: Lewis, Alexander M., Masgrau, Roser, Vasudevan, Sridhar R., Yamasaki, Michiko, O’Neill, John S., Garnham, Clive, James, Kristin, Macdonald, Andrew, Ziegler, Mathias, Galione, Antony, Churchill, Grant C.
Formato: Artigo
Lenguaje:Inglês
Publicado: Academic Press 2007
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2518627/
https://ncbi.nlm.nih.gov/pubmed/17919448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.08.030
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