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Selection of Respiratory Mutants of Neurospora crassa

A method is described that permits the isolation of mutants that are defective in mitochondrial respiration. The techniques of inositol-less death and overlay with 2,3,5-triphenyl-2H-tetrazolium chloride are utilized to select for mutant colonies. Colonies that survive inositol-less death and fail t...

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Bibliografske podrobnosti
Main Authors: Edwards, David L., Kwiecinski, Fabia, Horstmann, Jonathan
Format: Artigo
Jezik:Inglês
Izdano: 1973
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC251752/
https://ncbi.nlm.nih.gov/pubmed/4349028
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