Selection of Respiratory Mutants of Neurospora crassa

A method is described that permits the isolation of mutants that are defective in mitochondrial respiration. The techniques of inositol-less death and overlay with 2,3,5-triphenyl-2H-tetrazolium chloride are utilized to select for mutant colonies. Colonies that survive inositol-less death and fail t...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Edwards, David L., Kwiecinski, Fabia, Horstmann, Jonathan
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1973
Subjects:
Physiology and Metabolism
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC251752/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4349028/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.114.1.164-168.1973
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