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The positively charged C-terminal subdomains of EcoRV endonuclease: contributions to DNA binding, bending and cleavage
The carboxy-terminal subdomains of the homodimeric EcoRV restriction endonuclease each bear a net +4 charge, and are positioned on the inner concave surface of the 50° DNA bend that is induced by the enzyme. A complete kinetic and structural analysis of a truncated EcoRV mutant lacking these domains...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2515858/ https://ncbi.nlm.nih.gov/pubmed/16981705 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0606400 |
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