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EPR Spectroscopic and Computational Characterization of the Hydroxyethylidene-Thiamine Pyrophosphate Radical Intermediate of Pyruvate:Ferredoxin Oxidoreductase

The radical intermediate of pyruvate:ferredoxin oxidoreductase (PFOR) from Moorella thermoacetica was characterized using electron paramagnetic resonance (EPR) spectroscopy at X-band and D-band microwave frequencies. EPR spectra obtained with various combinations of isotopically labeled substrate (p...

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Detalhes bibliográficos
Main Authors: Mansoorabadi, Steven O., Seravalli, Javier, Furdui, Cristina, Krymov, Vladimir, Gerfen, Gary J., Begley, Tadhg P., Melnick, Jonathan, Ragsdale, Stephen W., Reed, George H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2505060/
https://ncbi.nlm.nih.gov/pubmed/16752902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0602516
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