cis- and trans-cleavage activities of poliovirus 2A protease expressed in Escherichia coli.

The poliovirus protease, 2Apro, was produced in Escherichia coli from plasmids that encode a fusion protein consisting of the N-terminal portion of the bacterial TrpE protein linked to poliovirus 2Apro. This fusion protein underwent efficient autocatalytic cleavage at the N terminus of 2Apro, genera...

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Argitaratua izan da:	J Virol
Egile Nagusiak:	Alvey, J C, Wyckoff, E E, Yu, S F, Lloyd, R, Ehrenfeld, E
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	American Society for Microbiology (ASM) 1991
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC250281/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1656087/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.11.6077-6083.1991
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