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cis- and trans-cleavage activities of poliovirus 2A protease expressed in Escherichia coli.

The poliovirus protease, 2Apro, was produced in Escherichia coli from plasmids that encode a fusion protein consisting of the N-terminal portion of the bacterial TrpE protein linked to poliovirus 2Apro. This fusion protein underwent efficient autocatalytic cleavage at the N terminus of 2Apro, genera...

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Bibliografiska uppgifter
Huvudupphovsmän: Alvey, J C, Wyckoff, E E, Yu, S F, Lloyd, R, Ehrenfeld, E
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1991
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC250281/
https://ncbi.nlm.nih.gov/pubmed/1656087
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