Isolation of Spheroplast Membranes and Stability of Spheroplasts of Bacillus stearothermophilus
Spheroplasts were prepared by lysozyme digestion of the cell wall and ruptured by suspension in 0.15 m NaCl, followed by centrifugation at 30,900 × g for 35 min, and by a final suspension in 0.05 m NaCl for 12 to 16 hr at 5 C. The membrane ghosts were washed four times in tris(hydroxylmethyl)aminome...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1969
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC249778/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5777801/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.97.2.924-935.1969 |
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