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Isolation of Spheroplast Membranes and Stability of Spheroplasts of Bacillus stearothermophilus

Spheroplasts were prepared by lysozyme digestion of the cell wall and ruptured by suspension in 0.15 m NaCl, followed by centrifugation at 30,900 × g for 35 min, and by a final suspension in 0.05 m NaCl for 12 to 16 hr at 5 C. The membrane ghosts were washed four times in tris(hydroxylmethyl)aminome...

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Bibliografski detalji
Glavni autori: Bodman, Hollis, Welker, N. E.
Format: Artigo
Jezik:Inglês
Izdano: 1969
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC249778/
https://ncbi.nlm.nih.gov/pubmed/5777801
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