Crystallization and preliminary X-ray diffraction analysis of biotin acetyl-CoA carboxylase ligase (BirA) from Mycobacterium tuberculosis

The gene encoding biotin acetyl-CoA carboxylase ligase (BirA) from Myco­bacterium tuberculosis was cloned and expressed in Escherichia coli with a C-­terminal Strep-tag. PEG 4000 as well as PEG 8000 were used as precipitants at pH 7.5 to crystallize the protein using the vapour-diffusion technique....

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Main Authors: Gupta, Vibha, Gupta, Rakesh K., Khare, Garima, Surolia, Avadhesha, Salunke, Dinakar M., Tyagi, Anil K.
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2008
Assuntos:
Crystallization Communications
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2496853/
https://ncbi.nlm.nih.gov/pubmed/18540066
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108012475
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