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Protocol to determine accurate absorption coefficients for iron containing transferrins
An accurate protein concentration is an essential component of most biochemical experiments. The simplest method to determine a protein concentration is by measuring the A(280), using an absorption coefficient (ε), and applying the Beer-Lambert law. For some metalloproteins (including all transferri...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2494952/ https://ncbi.nlm.nih.gov/pubmed/18471984 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.04.012 |
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