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Protocol to determine accurate absorption coefficients for iron containing transferrins

An accurate protein concentration is an essential component of most biochemical experiments. The simplest method to determine a protein concentration is by measuring the A(280), using an absorption coefficient (ε), and applying the Beer-Lambert law. For some metalloproteins (including all transferri...

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Hlavní autoři: James, Nicholas G., Mason, Anne B.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2494952/
https://ncbi.nlm.nih.gov/pubmed/18471984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.04.012
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