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Mapping of helicase and helicase substrate-binding domains on simian virus 40 large T antigen.

We generated fragments of simian virus 40 large tumor antigen (T antigen) by tryptic digestion and assayed them for helicase activity and helicase substrate (mostly single-stranded DNA)-binding activity in order to map the domain sites on the protein. The N-terminal 130 amino acids were not required...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wun-Kim, K, Simmons, D T
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1990
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC249356/
https://ncbi.nlm.nih.gov/pubmed/2157869
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