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The ribosome-in-pieces: Binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomal RNA

An oligoribonucleotide (a 27-mer) that mimics the sarcin/ricin (S/R) domain of Escherichia coli 23S rRNA binds elongation factor EF-G; the K(d) is 6.9 μM, whereas for binding to ribosomes it is 0.7 μM. Binding saturates when EF-G and the S/R RNA are equimolar; at saturation 70% of the input RNA is i...

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Autors principals: Munishkin, Alexander, Wool, Ira G.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences of the USA 1997
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC24907/
https://ncbi.nlm.nih.gov/pubmed/9356440
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