In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the small 6,000-molecular-weight membrane protein modulates virus release.

We report on the construction of a full-length cDNA clone of Semliki Forest virus (SFV). By placing the cDNA under the SP6 promoter, infectious RNA can be produced in vitro and used to transfect cells to initiate virus infection. To achieve efficient transfections, a new protocol for electroporation...

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Dades bibliogràfiques

Publicat a:	J Virol
Autors principals:	Liljeström, P, Lusa, S, Huylebroeck, D, Garoff, H
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1991
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC248843/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2072446/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.8.4107-4113.1991
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