In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the small 6,000-molecular-weight membrane protein modulates virus release.

We report on the construction of a full-length cDNA clone of Semliki Forest virus (SFV). By placing the cDNA under the SP6 promoter, infectious RNA can be produced in vitro and used to transfect cells to initiate virus infection. To achieve efficient transfections, a new protocol for electroporation...

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Detalles Bibliográficos

Publicado en:	J Virol
Principais autores:	Liljeström, P, Lusa, S, Huylebroeck, D, Garoff, H
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC248843/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2072446/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.8.4107-4113.1991
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