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Purification of human respiratory syncytial virus by ultracentrifugation in iodixanol density gradient

Ultracentrifugation in sucrose density gradient remains the most commonly used technique for hRSV purification. However, the high viscosity and hyper-osmotic property of sucrose can cause damage to the extremely labile virus leading to loss of infectivity. To overcome these limitations, an alternati...

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Autori principali: Gias, E., Nielsen, S.U., Morgan, L.A.F., Toms, G.L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier/North-Holland Biomedical Press 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2486346/
https://ncbi.nlm.nih.gov/pubmed/18029032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2007.09.013
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