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Purification of human respiratory syncytial virus by ultracentrifugation in iodixanol density gradient

Ultracentrifugation in sucrose density gradient remains the most commonly used technique for hRSV purification. However, the high viscosity and hyper-osmotic property of sucrose can cause damage to the extremely labile virus leading to loss of infectivity. To overcome these limitations, an alternati...

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Hlavní autoři: Gias, E., Nielsen, S.U., Morgan, L.A.F., Toms, G.L.
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier/North-Holland Biomedical Press 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2486346/
https://ncbi.nlm.nih.gov/pubmed/18029032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2007.09.013
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