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Chemical synthesis of the precursor molecule of the Aequorea green fluorescent protein, subsequent folding, and development of fluorescence

The present paper describes the total chemical synthesis of the precursor molecule of the Aequorea green fluorescent protein (GFP). The molecule is made up of 238 amino acid residues in a single polypeptide chain and is nonfluorescent. To carry out the synthesis, a procedure, first described in 1981...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Principais autores: Nishiuchi, Yuji, Inui, Tatsuya, Nishio, Hideki, Bódi, József, Kimura, Terutoshi, Tsuji, Frederick I., Sakakibara, Shumpei
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1998
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC24856/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9811837/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.23.13549
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