Specific Staining of Wall Mannan in Yeast Cells with Fluorescein-Conjugated Concanavalin A

A procedure is given for the coupling of fluorescein isothiocyanate to concanavalin A, a protein which specifically combines with a variety of polysaccharides, and for the subsequent isolation of the reactive conjugate. This fluorescent conjugate stains Saccharomyces cerevisiae but not Schizosacchar...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Tkacz, J. S., Cybulska, E. Barbara, Lampen, J. O.
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1971
Fag:	Morphology and Ultrastructure
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC248315/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5541005/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.105.1.1-5.1971
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