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Specific Staining of Wall Mannan in Yeast Cells with Fluorescein-Conjugated Concanavalin A

A procedure is given for the coupling of fluorescein isothiocyanate to concanavalin A, a protein which specifically combines with a variety of polysaccharides, and for the subsequent isolation of the reactive conjugate. This fluorescent conjugate stains Saccharomyces cerevisiae but not Schizosacchar...

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Bibliografische gegevens
Hoofdauteurs: Tkacz, J. S., Cybulska, E. Barbara, Lampen, J. O.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1971
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC248315/
https://ncbi.nlm.nih.gov/pubmed/5541005
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