Specific Staining of Wall Mannan in Yeast Cells with Fluorescein-Conjugated Concanavalin A

A procedure is given for the coupling of fluorescein isothiocyanate to concanavalin A, a protein which specifically combines with a variety of polysaccharides, and for the subsequent isolation of the reactive conjugate. This fluorescent conjugate stains Saccharomyces cerevisiae but not Schizosacchar...

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Bibliographic Details
Main Authors: Tkacz, J. S., Cybulska, E. Barbara, Lampen, J. O.
Format: Artigo
Language:Inglês
Published: 1971
Subjects:
Morphology and Ultrastructure
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC248315/
https://ncbi.nlm.nih.gov/pubmed/5541005
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