Specific Staining of Wall Mannan in Yeast Cells with Fluorescein-Conjugated Concanavalin A
A procedure is given for the coupling of fluorescein isothiocyanate to concanavalin A, a protein which specifically combines with a variety of polysaccharides, and for the subsequent isolation of the reactive conjugate. This fluorescent conjugate stains Saccharomyces cerevisiae but not Schizosacchar...
Uloženo v:
| Vydáno v: | J Bacteriol |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1971
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC248315/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5541005/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.105.1.1-5.1971 |
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