Regulation of Thymidine Metabolism in Escherichia coli K-12: Optimal Conditions for the Assay of 1,5-Phosphodeoxyribomutase in Ultrasonic Extracts

Assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of Escherichia coli K-12. The enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1,6-diphosphate for optimal activity. When cells are grown in minimal medium wi...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Bonney, Robert J., Weinfeld, Herbert
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1970
Schlagworte:	Genetics and Molecular Biology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC248139/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4919987/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.103.3.650-655.1970
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