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Regulation of Thymidine Metabolism in Escherichia coli K-12: Optimal Conditions for the Assay of 1,5-Phosphodeoxyribomutase in Ultrasonic Extracts
Assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of Escherichia coli K-12. The enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1,6-diphosphate for optimal activity. When cells are grown in minimal medium wi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1970
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC248139/ https://ncbi.nlm.nih.gov/pubmed/4919987 |
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