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A Fluorescence-Based Technique to Construct Size Distributions from Single-Object Measurements: Application to the Extrusion of Lipid Vesicles
We report a novel approach to quantitatively determine complete size distributions of surface-bound objects using fluorescence microscopy. We measure the integrated intensity of single particles and relate it to their size by taking into account the object geometry and the illumination profile of th...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2479610/ https://ncbi.nlm.nih.gov/pubmed/18424503 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.128819 |
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