Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli.
A cDNA clone encoding the RNA polymerase of poliovirus has been expressed in Escherichia coli under the transcriptional control of a T7 bacteriophage promoter. The poliovirus enzyme was designed to contain only a single additional amino acid, the N-terminal methionine. The recombinant enzyme has bee...
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| Опубликовано в:: | J Virol |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
1989
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC247675/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2535728/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.63.1.216-225.1989 |
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