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Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli.

A cDNA clone encoding the RNA polymerase of poliovirus has been expressed in Escherichia coli under the transcriptional control of a T7 bacteriophage promoter. The poliovirus enzyme was designed to contain only a single additional amino acid, the N-terminal methionine. The recombinant enzyme has bee...

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Detalles Bibliográficos
Main Authors: Plotch, S J, Palant, O, Gluzman, Y
Formato: Artigo
Idioma:Inglês
Publicado: 1989
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC247675/
https://ncbi.nlm.nih.gov/pubmed/2535728
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