Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA

The restriction endonuclease EcoRV can rapidly locate a short recognition site within long non-cognate DNA using ‘facilitated diffusion’. This process has long been attributed to a sliding mechanism, in which the enzyme first binds to the DNA via nonspecific interaction and then moves along the DNA...

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Detalhes bibliográficos
Main Authors: Bonnet, Isabelle, Biebricher, Andreas, Porté, Pierre-Louis, Loverdo, Claude, Bénichou, Olivier, Voituriez, Raphaël, Escudé, Christophe, Wende, Wolfgang, Pingoud, Alfred, Desbiolles, Pierre
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2008
Assuntos:
Nucleic Acid Enzymes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2475641/
https://ncbi.nlm.nih.gov/pubmed/18544605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn376
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