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α-Isopropylmalate Synthase from Yeast: Purification, Kinetic Studies, and Effect of Ligands on Stability

α-Isopropylmalate synthase, the first specific enzyme in leucine biosynthesis, was purified approximately 100-fold from extracts of Saccharomyces sp. (strain 60615), the most effective step being specific elution with the feedback inhibitor leucine from a hydroxyapatite column. In the early steps of...

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Hlavní autoři: Ulm, E. H., Böhme, R., Kohlhaw, G.
Médium: Artigo
Jazyk:Inglês
Vydáno: 1972
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC247535/
https://ncbi.nlm.nih.gov/pubmed/5079061
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