α-Isopropylmalate Synthase from Yeast: Purification, Kinetic Studies, and Effect of Ligands on Stability

α-Isopropylmalate synthase, the first specific enzyme in leucine biosynthesis, was purified approximately 100-fold from extracts of Saccharomyces sp. (strain 60615), the most effective step being specific elution with the feedback inhibitor leucine from a hydroxyapatite column. In the early steps of...

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Dettagli Bibliografici

Pubblicato in:	J Bacteriol
Autori principali:	Ulm, E. H., Böhme, R., Kohlhaw, G.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1972
Soggetti:	Enzymology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC247535/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5079061/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.110.3.1118-1126.1972
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