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Isolation of a Mutant of Escherichia coli Defective in Cytosine-Specific Deoxyribonucleic Acid Methylase Activity and in Partial Protection of Bacteriophage λ Against Restriction by Cells Containing the N-3 Drug-Resistance Factor

A mutant (designated mec(−)) of Escherichia coli F(+) 100 endo I(−)su(+) r(K)(−)m(K)(+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages λ and fd propagated in it, is virtually devoid of 5-m...

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Bibliographic Details
Main Authors: Hattman, Stanley, Schlagman, Samuel, Cousens, Lawrence
Format: Artigo
Language:Inglês
Published: 1973
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC246359/
https://ncbi.nlm.nih.gov/pubmed/4353870
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