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Gene 1.7 of bacteriophage T7 confers sensitivity of phage growth to dideoxythymidine
Bacteriophage T7 DNA polymerase efficiently incorporates dideoxynucleotides into DNA, resulting in chain termination. Dideoxythymidine (ddT) present in the medium at levels not toxic to Escherichia coli inhibits phage T7. We isolated 95 T7 phage mutants that were resistant to ddT. All contained a mu...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2453740/ https://ncbi.nlm.nih.gov/pubmed/18599435 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804164105 |
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