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Identification of protein–protein interfaces by decreased amide proton solvent accessibility

Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry was used to identify peptic fragments from protein complexes that retained deuterium under hydrogen exchange conditions due to decreased solvent accessibility at the interface of the complex. Short deuteration times allowed...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Mandell, Jeffrey G., Falick, Arnold M., Komives, Elizabeth A.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1998
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Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC24513/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9843953/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.25.14705
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