Substrate Specificity of the 3-Methylcrotonyl Coenzyme A (CoA) and Geranyl-CoA Carboxylases from Pseudomonas aeruginosa

Biotin-containing 3-methylcrotonyl coenzyme A (MC-CoA) carboxylase (MCCase) and geranyl-CoA (G-CoA) carboxylase (GCCase) from Pseudomonas aeruginosa were expressed as His-tagged recombinant proteins in Escherichia coli. Both native and recombinant MCCase and GCCase showed pH and temperature optima o...

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Detalhes bibliográficos
Main Authors: Aguilar, J. A., Díaz-Pérez, C., Díaz-Pérez, A. L., Rodríguez-Zavala, J. S., Nikolau, B. J., Campos-García, J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Enzymes and Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2447018/
https://ncbi.nlm.nih.gov/pubmed/18469096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00454-08
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