Automated and Manual Methods of DNA Extraction for Aspergillus fumigatus and Rhizopus oryzae Analyzed by Quantitative Real-Time PCR

Quantitative real-time PCR (qPCR) may improve the detection of fungal pathogens. Extraction of DNA from fungal pathogens is fundamental to optimization of qPCR; however, the loss of fungal DNA during the extraction process is a major limitation to molecular diagnostic tools for pathogenic fungi. We...

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Detalhes bibliográficos
Main Authors: Francesconi, Andrea, Kasai, Miki, Harrington, Susan M., Beveridge, Mara G., Petraitiene, Ruta, Petraitis, Vidmantas, Schaufele, Robert L., Walsh, Thomas J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Mycology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446852/
https://ncbi.nlm.nih.gov/pubmed/18353931
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02246-07
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