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Characterization of Detergent Purified Recombinant Rat Liver Monoamine Oxidase B Expressed in Pichia pastoris
The high level expression and purification of rat monoamine oxidase B (rMAOB) in the methylotrophic yeast Pichia pastoris is reported. Nearly 100 mg of purified rMAOB is obtained from 130 grams (wet weight) of cells (0.5 liter of culture). The MALDI-TOF mass spectrum of the purified protein shows a...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2445446/ https://ncbi.nlm.nih.gov/pubmed/18424170 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.03.002 |
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