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Nuclear receptor ligand-binding domains: reduction of helix H12 dynamics to favour crystallization

Crystallization trials of the human retinoid X receptor α ligand-binding domain (RXRα LBD) in complex with various ligands have been carried out. Using fluorescence anisotropy, it has been found that when compared with agonists these small-molecule effectors enhance the dynamics of the RXRα LBD C-­t...

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Detalhes bibliográficos
Main Authors: Nahoum, Virginie, Lipski, Alexandra, Quillard, Fabien, Guichou, Jean-François, Boublik, Yvan, Pérez, Efrèn, Germain, Pierre, de Lera, Angel R., Bourguet, William
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2443981/
https://ncbi.nlm.nih.gov/pubmed/18607089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108015492
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