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Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos

BACKGROUND: In vivo electroporation has been extensively used as an effective means of DNA transfer for analyzing gene function as well as gene regulation in developmental systems. In any of these two types of studies, the correct spatial and temporal expression of the electroporated transgene can o...

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Detalhes bibliográficos
Main Authors: Arede, Natacha, Tavares, Ana T.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2441435/
https://ncbi.nlm.nih.gov/pubmed/18612382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0002638
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