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Hydrogen-Deuterium Exchange Strategy for Delineation of Contact Sites in Protein Complexes
We use NMR spectra to determine protein-protein contact sites by observing differences in amide proton hydrogen-deuterium exchange in the complex compared to the free protein in solution. Aprotic organic solvents are used to preserve H/D labeling patterns that would be scrambled in water solutions....
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2440508/ https://ncbi.nlm.nih.gov/pubmed/18396165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.febslet.2008.03.043 |
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