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Tunable Blinking Kinetics of Cy5 for Precise DNA Quantification and Single-Nucleotide Difference Detection

Fluorescence correlation spectroscopy (FCS) can resolve the intrinsic fast-blinking kinetics (FBKs) of fluorescent molecules that occur on the order of microseconds. These FBKs can be heavily influenced by the microenvironments in which the fluorescent molecules are contained. In this work, FCS is u...

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Detalhes bibliográficos
Main Authors: Yeh, Hsin-Chih, Puleo, Christopher M., Ho, Yi-Ping, Bailey, Vasudev J., Lim, Teck Chuan, Liu, Kelvin, Wang, Tza-Huei
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2440434/
https://ncbi.nlm.nih.gov/pubmed/18424494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.127530
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