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Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies
We developed a strategy to introduce epitope tag–encoding DNA into endogenous loci by homologous recombination–mediated ‘knock-in’. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western b...
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| Главные авторы: | , , , , , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2008
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2435063/ https://ncbi.nlm.nih.gov/pubmed/18176569 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth1170 |
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