Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies

We developed a strategy to introduce epitope tag–encoding DNA into endogenous loci by homologous recombination–mediated ‘knock-in’. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western b...

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Autori principali: Zhang, Xiaodong, Guo, Chunguang, Chen, Yueting, Shulha, Hennady P, Schnetz, Michael P, LaFramboise, Thomas, Bartels, Cynthia F, Markowitz, Sanford, Weng, Zhiping, Scacheri, Peter C, Wang, Zhenghe
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2008
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2435063/
https://ncbi.nlm.nih.gov/pubmed/18176569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth1170
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