Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex.
Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the binding between the ligand and the hemagglutinin of the complex; the toxin is eluted selectively by dissoc...
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| Publicado en: | Appl Environ Microbiol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1978
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242946/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/655704/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.35.5.878-880.1978 |
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