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Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex.

Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the binding between the ligand and the hemagglutinin of the complex; the toxin is eluted selectively by dissoc...

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Bibliografiset tiedot
Julkaisussa:Appl Environ Microbiol
Päätekijät: Moberg, L J, Sugiyama, H
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology (ASM) 1978
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242946/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/655704/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.35.5.878-880.1978
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