Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation

Amyloid formation is cytotoxic and can activate the caspase cascade. Here, we monitor caspase-3-like activity as reduction of fluorescence resonance energy transfer (FRET) using the contstruct pFRET2-DEVD containing enhanced cyan fluorescent protin (EYFP) linked by the caspase-3 specific cleavage si...

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Detalhes bibliográficos
Main Authors: Paulsson, Johan F., Schultz, Sebastian W., Köhler, Martin, Leibiger, Ingo, Berggren, Per-Olof, Westermark, Gunilla T.
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2008
Assuntos:
Methodology Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2426809/
https://ncbi.nlm.nih.gov/pubmed/18566681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2008/865850
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