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Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway

I-SceI is a homing endonuclease that specifically cleaves an 18-bp double-stranded DNA. I-SceI exhibits a strong preference for cleaving the bottom strand DNA. The published structure of I-SceI bound to an uncleaved DNA substrate provided a mechanism for bottom strand cleavage but not for top strand...

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Bibliografski detalji
Glavni autori: Moure, Carmen M., Gimble, Frederick S., Quiocho, Florante A.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2008
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2425494/
https://ncbi.nlm.nih.gov/pubmed/18424798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn178
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