Use of magnesium to increase sensitivity of Limulus amoebocyte lysate for detection of endotoxin.
Increased sensitivity of the Limulus amoebocyte lysate (LAL) assay for the detection of endotoxin was attained by the reconstitution of commercially available LAL reagent with a magnesium-containing solution. As little as 2 to 6 pg (0.002 to 0.006 ng) of Escherichia coli O127:B8 endotoxin per ml was...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1983
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242461/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6859851/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.45.4.1342-1350.1983 |
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