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Use of magnesium to increase sensitivity of Limulus amoebocyte lysate for detection of endotoxin.

Increased sensitivity of the Limulus amoebocyte lysate (LAL) assay for the detection of endotoxin was attained by the reconstitution of commercially available LAL reagent with a magnesium-containing solution. As little as 2 to 6 pg (0.002 to 0.006 ng) of Escherichia coli O127:B8 endotoxin per ml was...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Tsuji, K, Steindler, K A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242461/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6859851/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.45.4.1342-1350.1983
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